Interferon gamma antibody

Catalog No. ABIN967468

This Mouse Monoclonal antibody specifically detects Interferon gamma in WB, FACS and ELISA (Detection). It exhibits reactivity toward Human.

Quick Overview for Interferon gamma antibody (ABIN967468)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA (Detection)

Clone: 4S-B3

Product Details anti-Interferon gamma Antibody

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Partially purified human IFN-gamma from supernatants of human PBMC stimulated with Staphylococcus aureus

Isotype: IgG1 kappa

Application Details

Application Notes: Blocking Control for Intracellular Staining:
The purified 4S.B3 antibody (ABIN967468) can be used as a blocking control to demonstrate specificity of IFN-gamma staining by directly conjugated clone 4S.B3. To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1-10 µg of purified 4S.B3 antibody (ABIN967468) for 20 minutes at 4°C, prior to staining with the directly conjugated antibody (e.g., 0.1 -0.5 µg mAb/1 million cells) (right panel). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

ELISA Detection:
The biotinylated 4S.B3 antibody is useful as a detection antibody in a sandwich ELISA for measuring human IFN-gamma protein levels. Biotinylated 4S.B3 antibody can be paired with the purified NIB42 antibody as the capture antibody, with recombinant human IFN-gamma as the standard. For testing human IFN-gamma complex in biological fluids like serum or plasma, our human IFN-gamma specific OptEIA™ sandwich ELISA set and OptEIA™ sandwich ELISA kit are recommended.

Western Blot:
The 4S.B3 antibody has been found useful for Western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml

Buffer: Aqueous buffered solution.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background: The 4S.B3 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate this hybridoma was partially purified human IFN-gamma obtained from supernatants of human PBMC stimulated with Staphylococcus aureus. This is a neutralizing antibody.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy