IL17F antibody

Catalog No. ABIN2689743

There are 9+ publications for this product available. The Mouse Monoclonal anti-IL17F antibody is suitable to detect IL17F in samples from Mouse. It has been validated for Neut and ICS.

Quick Overview for IL17F antibody (ABIN2689743)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL17F antibody is un-conjugated

Application: Neutralization (Neut), Intracellular Staining (ICS)

Clone: MM17F8F5

Product Details anti-IL17F Antibody

Characteristics: The MM17F8F5 monoclonal antibody specifically binds to Interleukin-17F (IL-17F) and can neutralize its biological activity. IL-17F is a member of the IL-17 family of cytokines. Among IL-17 family members, IL-17F has the highest amino acid sequence homology to IL-17A. IL-17F is produced by activated CD4+ T helper (Th17) cells, CD8+ T (Tc17) cells and γδ T cells. IL-17F can be secreted as homodimers or as heterodimers with IL-17A. IL-17F and IL-17A have overlapping functions such as inducing epithelial cells and fibroblasts to produce proinflammatory cytokines and chemokines including IL-6, GM-CSF, CXCL1, CCL2, and CCL7. These factors attract and activate neutrophils and other cell types that mediate protective responses against pathogenic microbes or pathologic allergic or autoimmune diseases. IL-17 gene knockout studies have shown that IL-17F and IL-17A have independent functions as well. IL-17F and IL-17A exert their biological function by binding to and signaling through IL-17 receptors comprised of the transmembrane receptor subunits, IL-17RA (CD217) and IL-17RC. Neutralization of mouse IL-17F biological activity with MM17F8F5, a Mouse Anti-Mouse IL-17F monoclonal antibody. In a 96-well microplate, biologically-active recombinant mouse IL-17F protein (0.2 μg/well) was preincubated (1 hr, 37 °C) with serial dilutions of Purified NA/LE Mouse Anti-Mouse IL-17F antibody (Cat. No. 563592, μg/mL) or tissue culture medium (as a control). Following the incubation, cells from the NIH-3T3 mouse embryonic cell line were added at 5 x 10^4 cells per well and were cultured at 37 °C. After 24 hours of culture, the supernatants from each well were harvested and their concentrations of IL-6 were quantified using a BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301). As the MM17F8F5 antibody was serially diluted, a neutralizing Anti-Mouse IL-17F antibody dose-response relationship was observed as shown in the figure. 563592 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified NA/LE Mouse Anti-Mouse IL-17F - NA/LE - Clone MM17F8F5 - Isotype Mouse IgG1, κ - Reactivity Ms - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: Sterile filtered,0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse IL-17F

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Without preservative

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL17F antibody (ABIN2689743)

Wilhelm, Hirota, Stieglitz, Van Snick, Tolaini, Lahl, Sparwasser, Helmby, Stockinger: "An IL-9 fate reporter demonstrates the induction of an innate IL-9 response in lung inflammation." in: Nature immunology, Vol. 12, Issue 11, pp. 1071-7, (2011) (PubMed).

Chang, Dong: "IL-17F: regulation, signaling and function in inflammation." in: Cytokine, Vol. 46, Issue 1, pp. 7-11, (2009) (PubMed).

Martinez, Zhang, Chung, Reynolds, Lin, Jetten, Feng, Dong: "Smad3 differentially regulates the induction of regulatory and inflammatory T cell differentiation." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35283-6, (2009) (PubMed).

Sutton, Lalor, Sweeney, Brereton, Lavelle, Mills: "Interleukin-1 and IL-23 induce innate IL-17 production from gammadelta T cells, amplifying Th17 responses and autoimmunity." in: Immunity, Vol. 31, Issue 2, pp. 331-41, (2009) (PubMed).

Dong: "Mouse Th17 cells: current understanding of their generation and regulation." in: European journal of immunology, Vol. 39, Issue 3, pp. 640-4, (2009) (PubMed).

Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).

Ishigame, Kakuta, Nagai, Kadoki, Nambu, Komiyama, Fujikado, Tanahashi, Akitsu, Kotaki, Sudo, Nakae, Sasakawa, Iwakura: "Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses." in: Immunity, Vol. 30, Issue 1, pp. 108-19, (2009) (PubMed).

Yang, Chang, Park, Nurieva, Shah, Acero, Wang, Schluns, Broaddus, Zhu, Dong: "Regulation of inflammatory responses by IL-17F." in: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1063-75, (2008) (PubMed).

Oda, Canelos, Essayan, Plunkett, Myers, Huang: "Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response." in: American journal of respiratory and critical care medicine, Vol. 171, Issue 1, pp. 12-8, (2004) (PubMed).

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL-17F

Background: Synonyms: Il17f, interleukin 17F, Interleukin-17F

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity