Ki-67 antibody

Catalog No. ABIN2689800

The Mouse Monoclonal anti-Ki-67 antibody has been validated for FACS, IHC (f) and IHC (fro). It is suitable to detect Ki-67 in samples from Human, Mouse, Rat and Pig. There are 2+ publications available.

Quick Overview for Ki-67 antibody (ABIN2689800)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B56

Product Details anti-Ki-67 Antibody

Brand: BD Pharmingen™

Characteristics: Recognizes Ki-67, a nuclear cell proliferation-associated antigen expressed in all active stages of the cell cycle. Ki-67 is revealed as a double band (345 and 395 kD) in western blot analysis of proliferating cells. B56 was developed using an immunogen composed of the immunodominant epitope of the Ki-67 protein. Antibodies B56 and MIB 1 react with this immunogen. Flow cytometric analysis reveals that the binding of B56-PE can be blocked by MIB 1 purified antibody. Immunohistochemistry analysis demonstrates a staining pattern similar to MIB 1 on both frozen and paraffin-embedded tissue sections. Immunohistochemistry of Ki-67 positive cells.

BD Pharmingen™ Purified Mouse Anti-Human Ki-67 - Purified - Clone B56 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms, Pig, Rat - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ki-67

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Ki-67 antibody (ABIN2689800)

Shi, Cui, Alam, Gunning, Nestor, Giovannucci, Zhang, Ding: "Nestin expression defines both glial and neuronal progenitors in postnatal sympathetic ganglia." in: The Journal of comparative neurology, Vol. 508, Issue 6, pp. 867-78, (2008) (PubMed).

Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: Synonyms: MKI67, Antigen identified by monoclonal antibody Ki-67, KIA

Pathways: Glycosaminoglycan Metabolic Process