CD207 antibody

Catalog No. ABIN2689803

The Mouse Monoclonal anti-CD207 antibody has been validated for IHC and ICS. It is suitable to detect CD207 in samples from Human. There are 5+ publications available.

Quick Overview for CD207 antibody (ABIN2689803)

Target: CD207 (CD207 Molecule, Langerin (CD207))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD207 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Intracellular Staining (ICS)

Clone: 2G3

Product Details anti-CD207 Antibody

Brand: BD Pharmingen™

Characteristics: The 2G3 monoclonal antibody specifically binds to Langerin which is also known as CD207 or C-type lectin domain family 4 member K (CLEC4K). CD207 is a 48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family. CD207 is selectively expressed by Langerhans cells, an immature dendritic cell type that is found in the epidermis and mucosal epithelia, and by some other dendritic cell subsets. CD207 is expressed on the cell surface membrane and by intracellular Birbeck granules whose formation depends on CD207. CD207 binds to certain surface carbohydrates that are expressed by microbes. It appears to play a role in the uptake of these microbes for subsequent processing and microbial antigen presentation to T cells. Immunohistochemical analysis of Langerin (CD207) expression by Langerhans cells within human skin. Acetone-fixed, frozen huma skin sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878, Left Image) or Purified Mouse Anti-Human Langerin (CD207) antibody (Cat. No. 564789, Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x.

BD Pharmingen™ Purified Mouse Anti-Human Langerin (CD207) - Purified - Clone 2G3 (also known as AB5_8.2G3.6 ) - Isotype Mouse IgG1, λ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Langerin Recombinant Protein

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD207 antibody (ABIN2689803)

Mayumi, Watanabe, Norose, Watari, Kawana, Geijtenbeek, Takahashi: "E-cadherin interactions are required for Langerhans cell differentiation." in: European journal of immunology, Vol. 43, Issue 1, pp. 270-80, (2013) (PubMed).

Flamar, Zurawski, Scholz, Gayet, Ni, Li, Klechevsky, Quinn, Oh, Kaplan, Banchereau, Zurawski: "Noncovalent assembly of anti-dendritic cell antibodies and antigens for evoking immune responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2645-55, (2012) (PubMed).

Igyártó, Haley, Ortner, Bobr, Gerami-Nejad, Edelson, Zurawski, Malissen, Zurawski, Berman, Kaplan: "Skin-resident murine dendritic cell subsets promote distinct and opposing antigen-specific T helper cell responses." in: Immunity, Vol. 35, Issue 2, pp. 260-72, (2011) (PubMed).

de Witte, Nabatov, Pion, Fluitsma, de Jong, de Gruijl, Piguet, van Kooyk, Geijtenbeek: "Langerin is a natural barrier to HIV-1 transmission by Langerhans cells." in: Nature medicine, Vol. 13, Issue 3, pp. 367-71, (2007) (PubMed).

Valladeau, Ravel, Dezutter-Dambuyant, Moore, Kleijmeer, Liu, Duvert-Frances, Vincent, Schmitt, Davoust, Caux, Lebecque, Saeland: "Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules." in: Immunity, Vol. 12, Issue 1, pp. 71-81, (2000) (PubMed).

Target Details for CD207

Target: CD207 (CD207 Molecule, Langerin (CD207))

Alternative Name: Langerin CD207

Background: Synonyms: CLC4K, CLEC4K, C-type lectin domain family 4 member K