Mast Cells antibody

Catalog No. ABIN2689832

This Mouse Monoclonal antibody specifically detects Mast Cells in FACS, IP, Sep and IHC. It exhibits reactivity toward Rat. It has been mentioned in 3+ publications

Quick Overview for Mast Cells antibody (ABIN2689832)

Target: Mast Cells

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Separation (Sep), Immunohistochemistry (IHC)

Clone: AR32AA4

Product Details

Characteristics: The AR32AA4 (AA4) antibody reacts with two distinct α-galactosyl derivatives of the ganglioside GD1b (disialogangliosides). The mAb AA4 reacts with four indistinct bands ranging in size from 56 - 110 kDa. Binding of the AA4 antibody to RBL-2H3 cells has been shown to inhibit IgE-mediated histamine release by inhibiting FcεR1 signal transduction events. The mAb AA4 has been shown to be useful for immunomagnetic mast cell separation from rat bone marrow and peritoneal lavage cell populations. This corresponds with reports that the AA4-binding epitope is expressed at higher levels than the FcεR1 on RBL-2H3 cells. Staining of mAb AR32AA4 on RBL (ATCC CRL-2256) cells. RBL cells were incubated with either Purified Mouse IgG1, κ isotype control (Cat. no. 557273, open dash line overlay) or purified AR32AA4 mAb (shaded histogram), followed by biotinylated F(ab')2 rat antimouse IgG and finally Streptavidin-FITC (Cat. no. 554060). The cells in both the shaded histogram and the overlay were gated on 7-AAD negative cells. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat Mast Cells - Purified - Clone AR32AA4 (also known as AA4) - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat basophilic leukemia cell line

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Jamur, Grodzki, Moreno, Swaim, Siraganian, Oliver: "Immunomagnetic isolation of rat bone marrow-derived and peritoneal mast cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 12, pp. 1715-22, (1998) (PubMed).

Stephan, Seibt, Dukanovic, Skasa, Swaim, Berenstein, Siraganian, Wahn: "Anti-ganglioside monoclonal antibody AA4 selectively inhibits IgE-induced signal transduction pathways in rat basophilic leukemia cells." in: Molecular immunology, Vol. 34, Issue 3, pp. 227-35, (1997) (PubMed).

Basciano, Berenstein, Kmak, Siraganian: "Monoclonal antibodies that inhibit IgE binding." in: The Journal of biological chemistry, Vol. 261, Issue 25, pp. 11823-31, (1986) (PubMed).

Target Details

Target: Mast Cells