Panendothelial Cell Antigen antibody

Catalog No. ABIN2689874

This Rat Monoclonal antibody specifically detects Panendothelial Cell Antigen in FACS, IHC (fro) and IHC (p). It exhibits reactivity toward Mouse. It has been mentioned in 3+ publications

Quick Overview for Panendothelial Cell Antigen antibody (ABIN2689874)

Target: Panendothelial Cell Antigen

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MECA

Product Details

Characteristics: The MECA-32 antibody reacts with a dimer of 50-55- kDa subunits expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of cardiac and skeletal muscle and the brain. Normally in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules, however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen. Immunohistochemical staining of mouse Panendothelial cell antigen: Frozen sections of normal mouse small intestine were reacted with the anti-panendothelial cell antigen monoclonal antibody. Cells expressing this molecule can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse Panendothelial Cell Antigen - Purified - Clone MECA-32 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse lymph node stromal cells

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Engelhardt, Conley, Butcher: "Cell adhesion molecules on vessels during inflammation in the mouse central nervous system." in: Journal of neuroimmunology, Vol. 51, Issue 2, pp. 199-208, (1994) (PubMed).

Bergese, Pelletier, Ohye, Vallera, Orosz: "Treatment of mice with anti-CD3 mAb induces endothelial vascular cell adhesion molecule-1 expression." in: Transplantation, Vol. 57, Issue 5, pp. 711-7, (1994) (PubMed).

Leppink, Bishop, Sedmak, Henry, Ferguson, Streeter, Butcher, Orosz: "Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration." in: Transplantation, Vol. 48, Issue 5, pp. 874-7, (1989) (PubMed).

Target Details

Target: Panendothelial Cell Antigen

Background: Synonyms: Plvap, Pv1, MECA32, Plasmalemma vesicle-associated protein