SIGLEC5 antibody

Catalog No. ABIN2689902

The Rat Monoclonal anti-SIGLEC5 antibody has been validated for ELISA, FACS, IHC (fro) and IF. It is suitable to detect SIGLEC5 in samples from Mouse. There are 2+ publications available.

Quick Overview for SIGLEC5 antibody (ABIN2689902)

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SIGLEC5 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: E50

Product Details anti-SIGLEC5 Antibody

Brand: BD Pharmingen™

Characteristics: The E50-2440 antibody reacts with Siglec-F, the sixth siglec protein to be reported in the mouse. Siglecs are the sialic acid-binding immunoglobulin superfamily lectins defined in the human, each of which has a distinctive expression pattern in the hematopoietic system and at least some of which are known to mediate cell-cell interactions. Orthologous proteins of human Siglec-1 (Sialoadhesin or CD169), Siglec-2 (CD22), and Siglec-4 (myelin-associated glycoprotein) have been characterized in the mouse. Human Siglec-3 (CD33) and Siglecs-5 through -10 are encoded by a cluster of closely related genes, and each has two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motifs). Similarly, mouse Siglec-F is encoded by a gene in a syntenic cluster in the mouse, and the protein has sialic acid-binding activity and an intracytoplasmic ITIM. Its expression pattern differs from those of the human Siglec-3-related proteins in that it is found on immature cells of the myelomonocytic lineage, with reduced expression on mature neutrophils and monocytes, and not on lymphoid cells. It has been proposed that mAb E50-2440 may be used for identification of immature myelomonocytic cells in the mouse. Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb E50-2440 (right panel) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-PE (Cat. No. 554061) and FITC-conjugated anti-mouse CD11b (Integrin αM chain) mAb M1/70 (Cat. No. 557396/553310). Flow cytometry was performed on a FACSCalibur™ .

BD Pharmingen™ Purified Rat Anti-Mouse Siglec-F - Purified - Clone E50-2440 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Siglec-F and human IgG Fc recombinant fusion protein

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SIGLEC5 antibody (ABIN2689902)

Angata, Hingorani, Varki, Varki: "Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45128-36, (2001) (PubMed).

Crocker, Varki: "Siglecs, sialic acids and innate immunity." in: Trends in immunology, Vol. 22, Issue 6, pp. 337-42, (2001) (PubMed).

Target Details for SIGLEC5

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Alternative Name: Siglec-F