SYK antibody (pTyr348) (PE)

Catalog No. ABIN2689913

This Mouse Monoclonal antibody specifically detects SYK in ICS. It exhibits reactivity toward Human and Mouse and has been mentioned in 7+ publications.

Quick Overview for SYK antibody (pTyr348) (PE) (ABIN2689913)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYK antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Clone: I120

Product Details anti-SYK Antibody (PE)

Binding Specificity: pTyr348

Brand: BD Phosflow™

Characteristics: Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral αβ TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igα and Igβ (CD79a and b), TCRζ, CD3ε, and FcRγ. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCγ, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342. Analysis of Syk (pY348) in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either stimulated (right panel) by cross-linking of surface IgM with purified Goat F(ab')2 Anti-Human IgM (SouthernBiotech) at 37 °C for 8 minutes or unstimulated (left panel). The cells were fixed with pre-warmed BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) for 10 minutes at 37 °C, permeabilized with BD Phosflow™ Perm Buffer II (Cat. No. 558052) at room temperature for 10 minutes, and then stained with PE Mouse anti-Syk (pY348) and Alexa Fluor® 647 Mouse anti-human CD20 mAb H1(FB1) (Cat. No. 558054). For data analysis, lymphocytes were selected by their scatter profile. The data demonstrates that the upregulated phosphorylation is restricted to the stimulated CD20-positive B lymphocytes. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Phosflow™ PE Mouse anti-Syk (pY348) - PE - Clone I120-722 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 50 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Syk Peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-SYK antibody (PE) (ABIN2689913)

Abtahian, Guerriero, Sebzda, Lu, Zhou, Mocsai, Myers, Huang, Jackson, Ferrari, Tybulewicz, Lowell, Lepore, Koretzky, Kahn: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, (2003) (PubMed).

Hong, Yankee, Harrison, Geahlen: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, (2002) (PubMed).

Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, (2002) (PubMed).

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).

Vines, Potter, Xu, Geahlen, Costello, Tybulewicz, Lowell, Chang, Gresham, Willman: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." in: Immunity, Vol. 15, Issue 4, pp. 507-19, (2001) (PubMed).

Coopman, Do, Barth, Bowden, Hayes, Basyuk, Blancato, Vezza, McLeskey, Mangeat, Mueller: "The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells." in: Nature, Vol. 406, Issue 6797, pp. 742-7, (2000) (PubMed).

Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling