Ly76 antibody (Biotin)

Catalog No. ABIN2689924

Cited in 3+ publications. The Biotin-conjugated Rat Monoclonal anti-Ly76 antibody (Clone TER-119) (ABIN2689924) specifically detects Ly76 in FACS and IHC. The antibody is reactive with Mouse samples.

Quick Overview for Ly76 antibody (Biotin) (ABIN2689924)

Target: Ly76 (Lymphocyte Antigen 76 (Ly76))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly76 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: TER-119

Product Details anti-Ly76 Antibody (Biotin)

Characteristics: The TER-119 antibody reacts with a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the "lineage cocktail" used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse TER-119/Erythroid Cells - Biotin - Clone TER-119 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Fetal Liver

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Ly76 antibody (Biotin) (ABIN2689924)

Kina, Ikuta, Takayama, Wada, Majumdar, Weissman, Katsura: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).

Kitajima, Kojima, Nakajima, Kondo, Hara, Miyajima, Takeuchi: "Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice." in: Blood, Vol. 93, Issue 1, pp. 87-95, (1999) (PubMed).

Ikuta, Kina, MacNeil, Uchida, Peault, Chien, Weissman: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).

Target Details for Ly76

Target: Ly76 (Lymphocyte Antigen 76 (Ly76))

Alternative Name: Lymphocyte Antigen 76

Background: Synonyms: Ly-76