TCR, V beta 8.1, 8.2 antibody (Biotin)

Catalog No. ABIN2689959

Product Details

Target: TCR, V beta 8.1, 8.2

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The MR5-2 antibody reacts with the Vβ 8.1 and Vβ 8.2 T-cell Receptors (TCR), but not the Vβ 8.3 TCR, of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 (Mls-1a, Mlsa), provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 (e.g., MA/MyJ), Mtv-44 (e.g., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Plate-bound MR5-2 antibody activates Vβ 8.1 or 8.2 TCR-bearing T lymphocytes. Two-color analysis of the expression of Vβ 8.1, 8.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated MR5-2, PE*conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 8.1, 8.2 TCR - Biotin - Clone MR5-2 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/6 mouse helper T-cell clone OI6

Clone: MR5

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry System.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Shinohara, Ikarashi, Maruoka, Miyata, Sugimura, Terada, Wakasugi: "Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains." in: European journal of immunology, Vol. 29, Issue 6, pp. 1871-8, (1999) (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Hugo, Kappler, Godfrey, Marrack: "Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1022-31, (1994) (PubMed).

Tomonari, Fairchild: "Positive and negative selection of Tcrb-V6+ T cells." in: Immunogenetics, Vol. 36, Issue 4, pp. 230-7, (1992) (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).

Target Details

Target: TCR, V beta 8.1, 8.2

Alternative Name: Vbeta 8.1, 8.2 TCR