pan Keratin antibody (AA 331-430) (Biotin)

Catalog No. ABIN2690219

The Rabbit Polyclonal anti-pan Keratin antibody has been validated for IHC (p) and IHC (fro). It is suitable to detect pan Keratin in samples from Human and Rat.

Quick Overview for pan Keratin antibody (AA 331-430) (Biotin) (ABIN2690219)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This pan Keratin antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-pan Keratin Antibody (Biotin)

Binding Specificity: AA 331-430

Specificity: This will target: CK4, CK75, CK3, CK2, CK6C, CK6A, keratin 6E

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK5

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin

Background:

Synonyms: CK4, CK75, CK3, CK2, CK6C, CK6A, keratin 6E, P-CK

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.