ErbB2/Her2 antibody (AA 676-1255)

Catalog No. ABIN2717953

This Mouse Monoclonal antibody specifically detects ErbB2/Her2 in WB, IHC, IF and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for ErbB2/Her2 antibody (AA 676-1255) (ABIN2717953)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 4F10

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: AA 676-1255

Characteristics: Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ErbB2/Her2 antibody (ABIN2717953)

Choi, de Poot, Lee, Kim, Han, Kim, Finley, Lee: "Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation." in: Nature communications, Vol. 7, pp. 10963, (2016) (PubMed).

Bian, Lin, Wu, Cui, Qi, Li, Su: "Metaplastic carcinoma of the breast: Imaging and pathological features." in: Oncology letters, Vol. 12, Issue 5, pp. 3975-3980, (2016) (PubMed).

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Molecular Weight: 137.7 kDa

Gene ID: 2064

NCBI Accession: NM_004448

HGNC: 2064

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development