TGFB3 antibody

Catalog No. ABIN271851

This anti-TGFB3 antibody is a Rabbit Polyclonal antibody detecting TGFB3 in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for TGFB3 antibody (ABIN271851)

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TGFB3 Antibody

Specificity: This antibody detects endogenous levels of pro-TGF-beta-3 (47 kDa) and cleaved-TGF-beta-3 (13 kDa) protein. (region surrounding Gly292)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for TGFB3

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Alternative Name: TGF-beta-3 (TGFB3)

Background: Transforming Growth Factor beta 3 (TGF beta 3) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional, capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors, and essentially all mammalian cells possess a specific TGF receptor. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. A high level of TGF beta 3 in normal adult heart, lung, and brain suggests a possible role for this factor in the regulation of a variety of normal physiological functions. The expression of TGF beta 3 in different locations and at specific stages of embryogenesis suggests its involvement in the regulation of development. Similar to the other isoforms of TGF beta, the upregulated activation of TGF beta 3 might also be involved in a variety of pathological conditions. TGF beta 3 has been detected in human, porcine, and avian sources and is expressed in cells of mesenchymal origin, suggesting a different role for this protein than for TGF beta 1 or TGF beta 2. TGF beta 3 is less prevalent in natural expression than either TGF beta 1 or TGF beta 2, but is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord and in several human carcinoma cells.Synonyms: Transforming growth factor beta-3

Molecular Weight: 13 and 47 kDa

Gene ID: 7043

NCBI Accession: NP_003230

UniProt: P10600

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus