ELISA: 1/5000-1/10000. Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACC-alpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACC-beta (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC-alpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACC-alpha, which maps to human chromosome 17, encodes the 265 kDa alpha form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACC-beta is homologous to that of ACC-alpha except for an additional peptide of about 150 amino acids at the N-terminus.Synonyms: ACAC, ACACA, ACC1, ACCA