DBT antibody

Catalog No. ABIN2719239

This Mouse Monoclonal antibody specifically detects DBT in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for DBT antibody (ABIN2719239)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 1G2

Product Details anti-DBT Antibody

Characteristics: Homo sapiens dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.

Isotype: IgG1

Application Details

Application Notes: WB 1:2000, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT

Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Molecular Weight: 46.4 kDa

Gene ID: 1629

NCBI Accession: NM_001918

HGNC: 1629