C5AR1 antibody

Catalog No. ABIN272007

The Rabbit Polyclonal anti-C5AR1 antibody (ABIN272007) specifically detects C5AR1 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for C5AR1 antibody (ABIN272007)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C5AR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-C5AR1 Antibody

Specificity: This antibody detects endogenous levels of D88/C5aR protein. (region surrounding Ser334)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: CD88 / C5R1

Background: The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

Molecular Weight: approx. 45 kDa

Gene ID: 728

NCBI Accession: NP_001727

UniProt: P21730

Pathways: Complement System