ERCC1 antibody

Catalog No. ABIN2720395

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Characteristics: Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human ERCC1 (NP_973730) produced in HEK293T cell.

Clone: 1H1

Isotype: IgG2a

Application Details

Application Notes: WB 1:500~2000, IF 1:100, FLOW 1:100

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Background: The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Molecular Weight: 32.4 kDa

Gene ID: 2067

NCBI Accession: NM_001983

HGNC: 2067

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response