PDE4D antibody

Catalog No. ABIN272057

The Rabbit Polyclonal anti-PDE4D antibody is suitable to detect PDE4D in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for PDE4D antibody (ABIN272057)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE4D Antibody

Specificity: Thhis antibody detects endogenous levels of PDE4D protein. (region surrounding His47)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background: Phosphodiesterases (PDE) hydrolyze cAMP to 5'AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4D gene maps to chromosome 5q12. The splice variants include isoforms PDE4D1-6.Synonyms: 5'-cyclic phosphodiesterase 4D, DPDE3, EC=3.1.4.17, PDE43, PDE4D, cAMP-specific 3'

Molecular Weight: approx. 88 kDa

Gene ID: 5144

NCBI Accession: NP_001098101

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling