PKD2 antibody

Catalog No. ABIN272069

The Rabbit Polyclonal anti-PKD2 antibody is suitable to detect PKD2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PKD2 antibody (ABIN272069)

Target: PKD2 (Protein Kinase D2 (PKD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PKD2 Antibody

Specificity: This antibody detects endogenous levels of PKD2 protein. (region surrounding Ala872)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PKD2

Target: PKD2 (Protein Kinase D2 (PKD2))

Alternative Name: PRKD2 / PKD2

Background: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.Synonyms: HSPC187, PKC-D2, Serine/threonine-protein kinase D2, nPKC-D2

Molecular Weight: approx. 96 kDa

Gene ID: 25865

NCBI Accession: NP_001073349

UniProt: Q9BZL6

Pathways: cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity