ALDOA antibody

Catalog No. ABIN272155

This Rabbit Polyclonal antibody specifically detects ALDOA in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALDOA antibody (ABIN272155)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDOA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ALDOA Antibody

Specificity: This antibody detects endogenous levels of Aldolase A protein. (region surrounding Thr9)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Bot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Background: Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldolase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2 % of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase Cnegative Purkinje cells.Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1

Molecular Weight: approx. 39 kDa

Gene ID: 226

NCBI Accession: NP_000025

UniProt: P04075

Pathways: Ribonucleoside Biosynthetic Process