PD-L1 antibody (PE)

Catalog No. ABIN2738460

The Mouse Monoclonal anti-PD-L1 antibody has been validated for FACS and ELISA. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for PD-L1 antibody (PE) (ABIN2738460)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), ELISA

Clone: ANC6H1

Product Details anti-PD-L1 Antibody (PE)

Specificity: Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.

Purification: Protein A purified

Immunogen: Recombinant human CD274(B7-H1).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. 5 x 10⁵ per tube were washed and pre incubated 5 minutes with 20^l of 250^g/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-human CD274 at a dilution factor of 1:50 (20 ^g/mL). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: B7-H1 / PD-L1 / CD274

Background: Name/Gene ID: CD274

Synonyms: CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 Molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1

Gene ID: 29126

Pathways: Cancer Immune Checkpoints