CA9 antibody

Catalog No. ABIN2738683

This anti-CA9 antibody is a Mouse Monoclonal antibody detecting CA9 in WB, IHC, ELISA, IF, FACS, IP and IHC (p). Suitable for Human and Horse.

Quick Overview for CA9 antibody (ABIN2738683)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 66-4-C2

Product Details anti-CA9 Antibody

Specificity: Human CA9 / Carbonic Anhydrase IX

Purification: Protein G purified

Immunogen: The microsomal fraction of human renal cortical tissue homogenate.

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a glycoprotein of ~200  kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9 / Carbonic Anhydrase IX

Background: Name/Gene ID: CA9

Synonyms: CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Carbonic dehydratase, G250, MN, Membrane antigen MN, p54/58N, RCC-associated antigen G250, RCC-associated protein G250, Carbonic anhydrase 9, PMW1

Gene ID: 768