CD34 antibody (N-Term)

Catalog No. ABIN2739286

This anti-CD34 antibody is a Mouse Monoclonal antibody detecting CD34 in FACS, WB, IHC, IF, IHC (p) and IP. Suitable for Human, Cynomolgus and Rhesus Monkey.

Quick Overview for CD34 antibody (N-Term) (ABIN2739286)

Target: CD34

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: QBEnd-10

Product Details anti-CD34 Antibody

Binding Specificity: N-Term

Specificity: Human CD34

No Cross-Reactivity: Cow (Bovine), Dog (Canine), Rat (Rattus), Sheep (Ovine)

Purification: Protein G purified

Immunogen: A detergent solubilized vesicular suspension prepared from human term placenta.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

Gene ID: 947