CD8 antibody (Cy7,PE)

Catalog No. ABIN2739653

This Mouse Monoclonal antibody specifically detects CD8 in FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD8 antibody (Cy7,PE) (ABIN2739653)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to Cy7,PE

Application: Flow Cytometry (FACS), ELISA

Clone: 14

Product Details anti-CD8 Antibody (Cy7,PE)

Specificity: Antibody 14 recognizes the CD8 alpha chain of approximately 33 kd (1).

Purification: Protein A purified

Immunogen: Mouse IgG1, kappa.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of antihuman CD8/PE-Cy7 at a 1:50 dilution (5 μg/mL). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 25.7 % sub population of the cells stained positive with a mean shift of 2.5 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-human CD8 antibody. ELISA: Use for detection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for CD8

Target: CD8

Alternative Name: CD8