CDK1 antibody

Catalog No. ABIN2739815

This Mouse Monoclonal antibody specifically detects CDK1 in WB, IHC, ELISA, IF, IP, IHC (p) and FACS. It exhibits reactivity toward Human, Cow, Monkey and Mink.

Quick Overview for CDK1 antibody (ABIN2739815)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Cow, Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: POH-1

Product Details anti-CDK1 Antibody

Specificity: Human CDK1 / CDC2

No Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Xenopus laevis, Mouse (Murine), Rat (Rattus), Yeast (Saccharomyces cerevisiae), Schizosaccharomyces pombe

Purification: Protein G purified

Immunogen: Purified recombinant human Cdk1 / p34cdc2.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (1 - 2 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a 34  kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. Cdk1 is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation. ELISA: Order BSA-free antibody format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 / CDC2

Background: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Gene ID: 983

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA