CEACAM8 antibody (Biotin)

Catalog No. ABIN2739905

This Mouse Monoclonal antibody specifically detects CEACAM8 in FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for CEACAM8 antibody (Biotin) (ABIN2739905)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM8 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: ANC1D5

Product Details anti-CEACAM8 Antibody (Biotin)

Specificity: Antibody from clone ANC1D5 binds to CD66b on the cell surface of human PMN in Flow cytometry and detects recombinant CD66b in sandwich EIA utilizing either clone ANC4A4 or ANC3C5 as a capture reagent.

Purification: Protein A purified

Immunogen: Recombinant soluble human CD66b.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20 μL of human IgG at 300 μg/mL (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 μL of anti-CD66b antibody(ANC1D5) at 10 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.66 log10 fluorescent units when compared to a Mouse IgG1/Biotin isotype control. Binding was blocked when cells were pre incubated with an excess of unlabeled anti-human CD66b (ANC1D5) mAb. ELISA: Use for detection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for CEACAM8

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CEACAM8 / CD66b

Background: Name/Gene ID: CEACAM8
Family: CEA

Synonyms: CEACAM8, CD66b antigen, Carcinoembryonic antigen CGM6, CD67, CD67 antigen, CGM6, NCA-95, CD66b

Gene ID: 1088