ESR2 antibody (AA 27-50)

Catalog No. ABIN2740734

The Rabbit Polyclonal anti-ESR2 antibody has been validated for WB. It is suitable to detect ESR2 in samples from Human.

Quick Overview for ESR2 antibody (AA 27-50) (ABIN2740734)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ESR2 Antibody

Binding Specificity: AA 27-50

Specificity: Human ESR2 / ER Beta

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Estrogen Receptor beta(27-50aa HGSIYIPSSYVDSHHEYPAMTFYS). Percent identity by BLAST analysis: Human, Gorilla (100%), Chimpanzee, Orangutan, Baboon, Monkey (96%), Gibbon, Marmoset (92%), Elephant (88%), Mouse, Rat, Cat, Bat (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 1 μg/mL)

Usage: Estrogen receptor-beta, referred to as ESR2, is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb. ESR2 is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells. ESR-beta is homologous to the previously identified ESR-alpha and has an overlapping but nonidentical tissue distribution. The DNA-binding domain of ESR-beta is 96 % conserved compared to ESR, and the ligand-binding domain shows 58 % conserved residues. ESR-beta is expressed in human thymus, spleen, ovary, and testis. Rat ESR-beta is expressed in rat prostate and ovary and is homologous to rat ESR(95 % conserved DNA-binding domain. 55 % conserved ligand-binding domain). ESR2 mRNA was coexpressed with ESR1 and its splice variants in 60 % of prolactinomas, 100 % of mixed growth hormone /prolactin tumors, and 29 % of gonadotroph tumors. ESR2 gene expression was not limited to ESR1-positive tumor subtypes, however, and was also found in 100 % of null cell tumors, 80 % of somatotroph tumors, and 60 % of corticotroph tumors.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2.5 % BSA, 0.025 % sodium azide/thimerosal.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2 / ER beta

Background: Name/Gene ID: ESR2
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

Gene ID: 2100

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling