ESR2 antibody (AA 201-300)

Catalog No. ABIN725210

Product Details anti-ESR2 Antibody

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ER-beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ESR2 antibody (ABIN725210)

Wei, Guo, Mu, Zhang, Zhao, Huai, Qiu, Li, Ma, Liu, Chen, Han: "Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 7, pp. 804-16, (2015) (PubMed).

Fang, Wu, Huang: "Raloxifene upregulated mesangial cell MMP-2 activity via ER-? through transcriptional regulation." in: Cell biochemistry and biophysics, Vol. 67, Issue 2, pp. 607-13, (2013) (PubMed).

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen receptor beta (ESR2 Products)

Synonyms: ER-BETA antibody, ESR-BETA antibody, ESRB antibody, ESTRB antibody, Erb antibody, NR3A2 antibody, ER[b]1 antibody, zfER-beta1 antibody, zfER[b]1 antibody, erb2 antibody, CERB antibody, ERB antibody, erb antibody, esrb antibody, estrb antibody, nr3a2 antibody, ERbeta antibody, er-beta antibody, esr-beta antibody, ESR2 antibody, ERBETA antibody, er antibody, ER-beta antibody, ER[b] antibody, Estrb antibody, ER[b]2 antibody, ER[b]a antibody, zfER[b]2 antibody, zfER-beta2 antibody, Erb2 antibody, esr2 antibody, erbeta antibody, Esr2 antibody, ERBeta2 antibody, estrogen receptor 2 antibody, estrogen receptor 2b antibody, estrogen receptor beta 2 antibody, estrogen receptor 2 (beta) antibody, estrogen receptor 2a antibody, estrogen receptor beta antibody, ESR2 antibody, esr2b antibody, erb2 antibody, esr2 antibody, Esr2 antibody, esr2a antibody, erbeta antibody

Background:

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Subcellular location: Nucleus

Synonyms: Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA, Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, ESR2

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling