HSP27 antibody

Catalog No. ABIN2741544

The Mouse Monoclonal anti-HSP27 antibody has been validated for WB, IHC, ELISA, IF, FACS, IP and IHC (p). It is suitable to detect HSP27 in samples from Human, Mouse, Rat, Sheep, Chimpanzee and Chicken.

Quick Overview for HSP27 antibody (ABIN2741544)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Mouse, Rat, Sheep, Chimpanzee, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: G3-1

Product Details anti-HSP27 Antibody

Specificity: Human HSPB1 / HSP27

Purification: Protein G purified

Immunogen: Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody recognizes a 24-27  kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced [Character 93]p29[Character 94] and [Character 93]24K[Character 94] protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 / HSP27

Background: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gene ID: 3315

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling