PDCD1LG2 antibody (Biotin)

Catalog No. ABIN2743535

The Mouse Monoclonal anti-PDCD1LG2 antibody has been validated for FACS, ELISA and BR. It is suitable to detect PDCD1LG2 in samples from Human.

Quick Overview for PDCD1LG2 antibody (Biotin) (ABIN2743535)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: ANC8D12

Product Details anti-PDCD1LG2 Antibody (Biotin)

Specificity: Antibody < span style="font-size: 11.0pt, mso-bidi-font-size: 10.0pt,">ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.

Purification: Protein A purified

Immunogen: Recombinant human CD273(B7-DC).

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Block, ELISA, Flo

Usage: Human monocyte derived Dendritic cells were prepared in 8 day culture. 5 x 10⁵ per tube were washed and incubated 45 minutes on ice with 80 μL of antihuman CD273/Biotin at concentration of 10 μg/mL. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PDCD1LG2 / CD273 / PD-L2

Background: Name/Gene ID: PDCD1LG2

Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2

Gene ID: 80380