PDCD1LG2 antibody (FITC)

Catalog No. ABIN2743537

This Mouse Monoclonal antibody specifically detects PDCD1LG2 in FACS, ELISA and BR. It exhibits reactivity toward Human.

Quick Overview for PDCD1LG2 antibody (FITC) (ABIN2743537)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: ANC8D12

Product Details anti-PDCD1LG2 Antibody (FITC)

Specificity: Antibody ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.

Purification: Protein A purified

Immunogen: Recombinant human CD273(B7-DC).

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Block, ELISA, Flo

Usage: Human monocyte derived Dendritic cells were prepared in 8 day culture. 5 x 10⁵ per tube were washed and incubated 45 minutes on ice with 80 μL of antihuman CD273/FITC at a 1:50 dilution (10 μg/mL). They were then washed twice and analyzed by FACS. Cells stained positive with a mean shift of 0.68 log10 fluorescent units when compared to isotype control Mouse IgG2a/FITC. Binding was blocked when reagent was pre incubated with a 10- fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PDCD1LG2 / CD273 / PD-L2

Background: Name/Gene ID: PDCD1LG2

Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2

Gene ID: 80380