EPH Receptor A5 antibody (AA 600-700)

Catalog No. ABIN2746568

This Rabbit Polyclonal antibody specifically detects EPH Receptor A5 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for EPH Receptor A5 antibody (AA 600-700) (ABIN2746568)

Target: EPH Receptor A5 (EPHA5)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EPH Receptor A5 Antibody

Binding Specificity: AA 600-700

Specificity: The antibody labels a strong band of EphAR5 at 124 kDa in ABIN1741641 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: EphA R5 selective antibodies were generated against a peptide taken from the human protein. The EphA R5-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The EphA R5- selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides EphA5 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for EphA R5 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 600-700 on human EphA R5 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for EPH Receptor A5

Target: EPH Receptor A5 (EPHA5)

Alternative Name: Eprhin Receptor A5

Background: The Ephrin receptors represent the largest group of Receptor Tyrosine Kinases, comprising of 14 members and divided in two subclasses (class A & B ephrin ligands) based on their abilities to bind and activate each other, and on sequence conservation. Ephrin-A (EFNA) class is anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) classes are trans-membrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrins interact with a variety of membrane receptors that respond to chemokines, neurotransmitters or growth factors. Eph receptors are involved in central nervous system function and development, and in the modulation of different types of nociception. Eph receptors and their ligands play important roles in the regulation of cancer cell migration and invasion and are key regulators of axon guidance. They function in a variety of signaling modes by transducing signals from the cell exterior to the interior through ligand-induced activation of their kinase domain. Ephrin A5, a member of the ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. Ephrin-A5 is highly expressed in the developing nervous system in several brain regions including the olfactory bulb, frontal cortex, striatum and hypothalamus. Ephrin-A5 acts as a guidance molecule regulating the trajectory of the ascending midbrain dopaminergic pathways. Ephrin- A5 expression is critical for proper development of central monoaminergic pathways and that its loss results in various neurodevelopmental abnormalities. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and mediate developmental events in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. EphrinA5 possesses two alternative isoforms, large ephrinA5 isoform (ephrinA5L) and small ephrinA5 isoform (ephrinA5S). EphrinA5L is a putative tumor suppressor in several types of human cancers and ephrin A5S, acts as a tumor suppressor specifically in human hepatocellular carcinomas (HCC).The gene for EphA R5 is present on chromosome 5q21

NCBI Accession: NP_004430

UniProt: P54756

Pathways: RTK Signaling