EPH Receptor A4 antibody (phosphorylated)

Catalog No. ABIN2747231

This Rabbit Polyclonal antibody specifically detects EPH Receptor A4 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for EPH Receptor A4 antibody (phosphorylated) (ABIN2747231)

Target: EPH Receptor A4 (EPHA4)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EPH Receptor A4 Antibody

Binding Specificity: AA 570-630, phosphorylated

Specificity: The antibody labels a strong band of PEphAR7 at 118 kDa in ABIN1741661 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: Phospho-specific EphAR4 selective antibodies were generated against a peptide taken from the human protein. The Phospho-specific EphAR4-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The PEphAR4-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides PEphA4 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for PEphAR4 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 570-630 on human EPHA R4 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Ephrin Type A receptor 4 antibodies/SEK/Tyrosine protein kinase MPK3/SEK1/EPHA4 Antibodies

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: Ephrin Receptor A4

Background: The Ephrin receptors represent the largest group of Receptor Tyrosine Kinases, comprising of 14 members and divided in two subclasses (class A & B ephrin ligands) based on their abilities to bind and activate each other, and on sequence conservation. Ephrin-A (EFNA) class is anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) classes are trans-membrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrins interact with a variety of membrane receptors that respond to chemokines, neurotransmitters or growth factors. Eph receptors are involved in central nervous system function and development, and in the modulation of different types of nociception. Eph receptors and their ligands play important roles in the regulation of cancer cell migration and invasion and are key regulators of axon guidance. They function in a variety of signaling modes by transducing signals from the cell exterior to the interior through ligand-induced activation of their kinase domain. Ephrin A4 receptor, a member of the ephrin family, binds both subclasses of Ephrins. EphA4 is the most abundant ephrin receptor in the nervous system. Ephrin- A4 / EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. This transcript variant, ephrin-A4, uses an alternate splice site in the 3' coding region that results in a downstream translation termination. EPH and EPH-related receptors are implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Motor recovery after photo thrombotic stroke is markedly enhanced in transgenic mice with reduced levels of EphA4, whereas infarct sizes are similar compared with non-transgenic controls. Pharmacological inhibition of the EphA4 signaling cascade using two ROCK (Rho-associated kinase), inhibitors,Y-27632 and fasudil, improved motor function of mice after stroke implying that reduction of EphA4 improves motor function after experimental stroke. Thus ROCK inhibition is a promising therapeutic strategy to enhance recovery after ischemic stroke. The gene for EphAR4 is present on chromosome 2q36.1

UniProt: Q03137

Pathways: RTK Signaling