Ki-67 antibody (N-Term)

Catalog No. ABIN2747277

The Rabbit Polyclonal anti-Ki-67 antibody has been validated for WB, ELISA and IP. It is suitable to detect Ki-67 in samples from Human, Mouse and Rat.

Quick Overview for Ki-67 antibody (N-Term) (ABIN2747277)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Ki-67 Antibody

Binding Specificity: N-Term

Specificity: The antibody labels a strong band of Ki67 at 395 kDa in ABIN1741685 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: Ki67 selective antibodies were generated against a peptide taken from the human protein. The Ki67-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The Ki67-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides Ki67 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for Ki67 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: N-epitope synthetic peptide corresponding to unique amino acid sequence on human Ki67 antigen.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67

Background: Ki-67 is a nuclear protein that is expressed in proliferating cells, is required for maintaining cell proliferation and is absent in quiescent cells. This non-histone protein was named Ki67 after the researcher's location, Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. Antigen Ki- 67, encoded by the MKI67 gene is expressed as two isoforms with molecular weights of 395 kDa and 345 kDa. Its main function is to organize and maintain the architecture of DNA and in the synthesis of ribosomes during mitosis. Ki-67 is a marker of cell proliferation and is detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in G0 phase. Thus, Ki- 67 is used as a marker for cell proliferation of solid tumors and some hematological malignancies. It is associated with ribosomal RNA transcription and inactivation of antigen Ki-67 leads to inhibition of ribosomal RNA synthesis. Ki-67 expression is used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. It is also important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 is used as prognostic/predictive markers in breast cancer and other malignant diseases, so high levels of Ki-67 are associated with poorer breast cancer survival. After a myocardial infarction, Ki-67 can be used to study myocyte proliferation, as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies. A correlation between tumor proliferation and disease progression has been observed with Ki-67 expression in renal and ureter tumors thus making it possible to differentiate high-risk patients. Ki-67 protein is a new member of the family of MPM-2 reactive phosphoproteins, which includes both structural and functional proteins that are necessary for the control and timing of mitosis. Phosphorylation and dephosphorylation of the Ki- 67 protein are controlled by key regulatory structures of the cell cycle and occur at two hallmark events within the cell cycle: the breakdown and the reorganization of the nucleus during mitosis. The human Ki67 gene maps to chromosome 10q26.2 and encodes a 3256 amino acid protein.

NCBI Accession: NP_002408

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process