ERG antibody (N-Term)

Catalog No. ABIN2747732

This Rabbit Polyclonal antibody specifically detects ERG in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERG antibody (N-Term) (ABIN2747732)

Target: ERG (ERG, ETS transcription factor (ERG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-ERG Antibody

Binding Specificity: N-Term

Specificity: The antibody labels a strong band of ERG at 58 kDa in ABIN1741673 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: ERG selective antibodies were generated against a peptide taken from the human protein. The ERG-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The ERG-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides ERG Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for ERG antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: N-epitope synthetic peptide corresponding to unique amino acid region on human ERG protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Transforming protein ERG, Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody, p55 antibody,TMPRSS2/ERG fusion antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Alternative Name: ERG

Background: Ets-Related Gene (ERG) product is a ~58 kDa nuclear protein which functions as a DNA-binding transcriptional regulator and belongs to the erythroblast transformation-specific (ETS) family of transcription factors. It is a nuclear and cytoplasmic transcriptional regulator that undergoes alternative splicing to produce multiple isoforms, designated Erg-1, Erg-2 and Erg-3. ERG plays an important role in cell development, differentiation,proliferation, apoptosis and tissue remodeling.This family of transcription factors contains approximately 30 members that share a highly conserved DNA-binding domain (ETS domain) and differs from each other in other domains (such as absence or presence of the Pointed/SAM domain). ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of proteins such as metalloprotease-1 (MMP-1) and MMP-3. ERG protein promotes the transcriptional activation of oncogenes and facilitates cancer progression. ERG is associated with prostatic intraepithelial neoplasia (PIN) and benign and malignant vascular endothelial cells in various different vascular tumors. ERG oncoprotein expression is a highly specific marker for prostate cancer as it is present in 50-70 % of all prostate carcinomas. Defects in ERG are a cause of Ewing sarcoma which is a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. Obesity is linked with poorer prostate cancer prognosis primarily in men with tumors harboring the gene fusion TMPRSS2: ERG. The human ERG gene maps to chromosome 21 and encodes a 486 amino acid protein.

UniProt: P11308