TNFRSF12A antibody

Catalog No. ABIN2749025

This Mouse Monoclonal antibody specifically detects TNFRSF12A in WB, FACS, IHC (fro) and Func. It exhibits reactivity toward Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for TNFRSF12A antibody (ABIN2749025)

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF12A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: ITEM-4

Product Details anti-TNFRSF12A Antibody

Purpose: Anti-Hu CD266 Purified Low Endotoxin

Specificity: The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: human CD266-transfected P815 cells

Isotype: IgG2b

Application Details

Application Notes: Functional application: Blocking.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNFRSF12A antibody (ABIN2749025)

Yoriki, Akashi, Sho, Nomi, Yamato, Hotta, Takayama, Matsumoto, Wakatsuki, Migita, Yagita, Nakajima: "Therapeutic potential of the TWEAK/Fn14 pathway in intractable gastrointestinal cancer." in: Experimental and therapeutic medicine, Vol. 2, Issue 1, pp. 103-108, (2012) (PubMed).

Roos, Wicovsky, Müller, Salzmann, Rosenthal, Kalthoff, Trauzold, Seher, Henkler, Kneitz, Wajant: "Soluble and transmembrane TNF-like weak inducer of apoptosis differentially activate the classical and noncanonical NF-kappa B pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 3, pp. 1593-605, (2010) (PubMed).

Nakayama, Ishidoh, Kojima, Harada, Kominami, Okumura, Yagita: "Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 341-8, (2002) (PubMed).

Target Details for TNFRSF12A

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Alternative Name: CD266

Background: TNF receptor superfamily member 12A,CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.,TWEAKR, FN14, TNFRSF12A

Gene ID: 51330

UniProt: Q9NP84

Pathways: Apoptosis, Regulation of Cell Size