TCR Vbeta5.3-Related antibody

Catalog No. ABIN2749039

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS, IP, WB and Func. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for TCR Vbeta5.3-Related antibody (ABIN2749039)

Target: TCR Vbeta5.3-Related

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Clone: MEM-262

Product Details

Purpose: Anti-Hu TCR Vbeta5.3-related Purified Low Endotoxin

Specificity: The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Cross-Reactivity (Details): Other not tested, Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human thymoma cell line HPB-ALL.

Isotype: IgG2a

Application Details

Application Notes: Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Functional application: This antibody activates T cells (V beta 5-related subset).
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Pavlistová, Drbal, Hilgert, Horejsí: "A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).

Target Details

Target: TCR Vbeta5.3-Related

Background: TCRVB5.3-related