CD161 antibody (PE)

Catalog No. ABIN2749047

Product Details anti-CD161 Antibody (PE)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purpose: Anti-Rt CD161 PE

Specificity: The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.

Cross-Reactivity (Details): Rat

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Splenic cells purified from the LEW rat

Clone: 10-78

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD161 antibody (PE) (ABIN2749047)

Chang, Tai, Roffler, Hwang: "The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 6025-32, (2004) (PubMed).

Stephens, Barclay, Mason: "Phenotypic characterization of regulatory CD4+CD25+ T cells in rats." in: International immunology, Vol. 16, Issue 2, pp. 365-75, (2004) (PubMed).

May, Dorris, Satumtira, Iqbal, Rehman, Lightfoot, Taurog: "CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 2, pp. 1099-105, (2003) (PubMed).

Tliba, Chauvin, Le Vern, Boulard, Sbille: "Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats." in: Veterinary research, Vol. 33, Issue 3, pp. 327-32, (2002) (PubMed).

Sedgwick, Ford, Foulcher, Airriess: "Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 11, pp. 5320-30, (1998) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161 (KLRB1 Products)

Background: Killer cell lectin-like receptor subfamily B, memb,CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.,NKRP1A, Klrb

Gene ID: 689817

UniProt: Q0ZUP0