Interferon gamma antibody (PE)

Catalog No. ABIN2749151

This Mouse Monoclonal antibody specifically detects Interferon gamma in ICFC. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 4+ publications.

Quick Overview for Interferon gamma antibody (PE) (ABIN2749151)

Target: Interferon gamma (IFNG)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: 4S-B3

Product Details anti-Interferon gamma Antibody (PE)

Purpose: Anti-IFN gamma PE

Specificity: The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Interferon gamma derived from human leukocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-Interferon gamma antibody (PE) (ABIN2749151)

Brincks, Kucaba, Legge, Griffith: "Influenza-induced expression of functional tumor necrosis factor-related apoptosis-inducing ligand on human peripheral blood mononuclear cells." in: Human immunology, Vol. 69, Issue 10, pp. 634-46, (2008) (PubMed).

Janke, Witsch, Mages, Hutloff, Kroczek: "Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells." in: Immunology, Vol. 118, Issue 3, pp. 353-60, (2006) (PubMed).

Coles, Wing, Smith, Coraddu, Greer, Taylor, Weetman, Hale, Chatterjee, Waldmann, Compston: "Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis." in: Lancet (London, England), Vol. 354, Issue 9191, pp. 1691-5, (1999) (PubMed).

Caulfield, Fernandez, Sousa, Lane, Lee, Hawrylowicz: "Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids." in: Immunology, Vol. 98, Issue 1, pp. 104-10, (1999) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma

Background: Interferon gamma,The interferon gamma (IFN-gamma, 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12, its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers, 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.,Interferon gamma, IFN-gamma

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy