CD8 alpha antibody

Catalog No. ABIN2749187

This Rat Monoclonal antibody specifically detects CD8 alpha in FACS, IP, IHC (fro), IHC (p) and ICC. It exhibits reactivity toward Mouse and has been mentioned in 8+ publications.

Quick Overview for CD8 alpha antibody (ABIN2749187)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: 53-6-7

Product Details anti-CD8 alpha Antibody

Purpose: Anti-Ms CD8a Purified

Specificity: The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa, alpha chain of the CD8 antigen).

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Murine spleen cells

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1.5 μg/mL.
Immunohistochemistry (frozen sections): Recommended dilution: 1:1000, formaldehyde fixation is not recommended, acetone fixation is preferred.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD8 alpha antibody (ABIN2749187)

Mochimaru, Usui, Yaguchi, Nagahama, Hasegawa, Usui, Shimmura, Tsubota, Amano, Kawakami, Ishida: "Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2." in: Investigative ophthalmology & visual science, Vol. 49, Issue 5, pp. 2172-7, (2008) (PubMed).

Bouwer, Alberti-Segui, Montfort, Berkowitz, Higgins: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, (2006) (PubMed).

Kamimura, Sawa, Sato, Agung, Hirano, Murakami: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, (2006) (PubMed).

Hata, Sakaguchi, Yoshitomi, Iwakura, Sekikawa, Azuma, Kanai, Moriizumi, Nomura, Nakamura, Sakaguchi: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, (2004) (PubMed).

Grabbe, Varga, Beissert, Steinert, Pendl, Seeliger, Bloch, Peters, Schwarz, Sunderkötter, Scharffetter-Kochanek: "Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells." in: The Journal of clinical investigation, Vol. 109, Issue 2, pp. 183-92, (2002) (PubMed).

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, (1992) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: CD8a molecule,The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

Gene ID: 12525

UniProt: P01731

Pathways: TCR Signaling