Ki-67 antibody

Catalog No. ABIN2749188

The Mouse Polyclonal anti-Ki-67 antibody has been validated for IHC, WB, FACS and ICC. It is suitable to detect Ki-67 in samples from Human and Cow. There are 3+ publications available.

Quick Overview for Ki-67 antibody (ABIN2749188)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human, Cow

Host: Mouse

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Ki-67 Antibody

Purpose: Anti-Ki-67 Purified

Specificity: The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Cross-Reactivity (Details): Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Nuclei of the Hodgkin lymphoma cell line L428

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation, recommended antibody concentration 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Ki-67 antibody (ABIN2749188)

Schlüter, Duchrow, Wohlenberg, Becker, Key, Flad, Gerdes: "The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins." in: The Journal of cell biology, Vol. 123, Issue 3, pp. 513-22, (1993) (PubMed).

Gerdes, Lemke, Baisch, Wacker, Schwab, Stein: "Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1710-5, (1984) (PubMed).

Gerdes, Schwab, Lemke, Stein: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, (1983) (PubMed).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: Marker of proliferation Ki-67,Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.,KIA, MIB, Marker of proliferation Ki67, MKI67

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process