FAS antibody

Catalog No. ABIN2749190

This Mouse Monoclonal antibody specifically detects FAS in FACS and Func. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for FAS antibody (ABIN2749190)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: EOS9-1

Product Details anti-FAS Antibody

Purpose: Anti-Hu CD95 Purified Low Endotoxin

Specificity: The mouse monoclonal antibody EOS9.1 recognizes an extracellular epitope of CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.

Cross-Reactivity (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: P815 cells transfected with human CD95

Isotype: IgM kappa

Application Details

Application Notes: Functional application: In vitro induction of apoptosis.
Flow cytometry: Recommended dilution: 2-6 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-FAS antibody (ABIN2749190)

Matsuoka, Kim, McDonough, Bascug, Warshauer, Koreth, Cutler, Ho, Alyea, Antin, Soiffer, Ritz: "Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation." in: The Journal of clinical investigation, Vol. 120, Issue 5, pp. 1479-93, (2010) (PubMed).

Kasper, Konze, Kern, Stippel: "CD95 and TNFα-induced apoptosis in liver metastases of colorectal carcinoma." in: In vivo (Athens, Greece), Vol. 24, Issue 5, pp. 653-7, (2010) (PubMed).

Conejo-Garcia, Benencia, Courreges, Gimotty, Khang, Buckanovich, Frauwirth, Zhang, Katsaros, Thompson, Levine, Coukos: "Ovarian carcinoma expresses the NKG2D ligand Letal and promotes the survival and expansion of CD28- antitumor T cells." in: Cancer research, Vol. 64, Issue 6, pp. 2175-82, (2004) (PubMed).

Desbarats, Birge, Mimouni-Rongy, Weinstein, Palerme, Newell: "Fas engagement induces neurite growth through ERK activation and p35 upregulation." in: Nature cell biology, Vol. 5, Issue 2, pp. 118-25, (2003) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95

Background: Fas cell surface death receptor,CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.,FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity