Cadherin 5 antibody

Catalog No. ABIN2749192

This anti-Cadherin 5 antibody is a Mouse Monoclonal antibody detecting Cadherin 5 in WB, IP, FACS, ICC and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Cadherin 5 antibody (ABIN2749192)

Target: Cadherin 5 (CDH5)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 55-7H1

Product Details anti-Cadherin 5 Antibody

Purpose: Anti-Hu CD144 Purified

Specificity: The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human endothelial cells

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Cadherin 5 antibody (ABIN2749192)

Hamilton, Foss, Leach: "Establishment of a human in vitro model of the outer blood-retinal barrier." in: Journal of anatomy, Vol. 211, Issue 6, pp. 707-16, (2007) (PubMed).

Limaye, Li, Hahn, Xia, Berndt, Vadas, Gamble: "Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members." in: Blood, Vol. 105, Issue 8, pp. 3169-77, (2005) (PubMed).

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: CD144

Background: Cadherin 5,CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.,Cadherin 5, CDH5, 7B4, VE-cadherin

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2