CD42b antibody

Catalog No. ABIN2752000

This anti-CD42b antibody is a Mouse Monoclonal antibody detecting CD42b in FACS, WB, ELISA, IP and ICC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD42b antibody (ABIN2752000)

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42b antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: AK2

Product Details anti-CD42b Antibody

Purpose: Anti-Hu CD42b Purified

Specificity: The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c, 160 kDa), CD42b/c forms a noncovalent complex with CD42a and CD42d.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human platelets

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD42b antibody (ABIN2752000)

Welsh, Colace, Muthard, Stalker, Brass, Diamond: "Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse." in: Journal of thrombosis and haemostasis : JTH, Vol. 10, Issue 11, pp. 2344-53, (2014) (PubMed).

Vettore, Scandellari, Moro, Lombardi, Scapin, Randi, Fabris et al.: "Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome ..." in: Haematologica, Vol. 93, Issue 11, pp. 1743-7, (2008) (PubMed).

Target Details for CD42b

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Alternative Name: CD42b

Background: Glycoprotein Ib platelet subunit alpha,CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.,BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3

Gene ID: 2811

UniProt: P07359