MBP
Reactivity: E. coli
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Cy5.5
Restrictions
For Research Use only
Format
Liquid
Buffer
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4
Preservative
ProClin
Precaution of Use
This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C/-80 °C
Target
Maltose Binding Protein (MBP)
Alternative Name
maltose Binding Protein
Background
Maltose-Binding Protein (MBP) is a native E. coli protein and is part of a complex responsible for the uptake and efficient catabolism of maltodextrins. MBP is composed of 370 amino acid residues. As a fusion tag, MBP is useful for recombinant protein purification by affinity chromatography. It is also used to increase the expression level and solubility of the tagged protein, and for promoting proper folding of the attached protein. MBP increases solubility and the tag is thus particularly useful for recombinant proteins that may otherwise accumulate in an insoluble forms as inclusion bodies. Affinity purification takes place under physiological conditions and mild elution is performed using maltose. The mild elution conditions preserve the activity of the MBP-tagged protein.