TPBG antibody

Catalog No. ABIN2763610

This Rabbit Polyclonal antibody specifically detects TPBG in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TPBG antibody (ABIN2763610)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPBG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TPBG Antibody

Cross-Reactivity: Human

Characteristics: Trophoblast glycoprotein, TPBG, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, M6P1,5T4 is a N-glycosylated transmembrane 72 kDa (420 AA) glycoprotein containing seven leucine-rich repeat regions. 5T4 is often referred to as an oncofetal antigen due to its expression in fetal trophoblast where it was first discovered or trophoblast glycoprotein (TPBG). 5T4 oncofetal antigen is defined by a monoclonal antibody originally raised against human placental trophoblast and which recognizes a 72 kD glycoprotein expressed in a variety of different carcinomas but detected only at very low levels in normal epithelia. Patterns of expression of 5T4 oncofetal antigen in colorectal carcinomas indicate a significant association with tumor metastasis. By IHC, 71 % of the carcinomas demonstrate positive 5T4 immunoreactivity in adenocarcinoma cells and/or associated stromal tissue.5T4 is found in tumors including the colorectal, ovarian, and gastric. Its expression is used as a prognostic aid in these cases. 5T4 is widespread in malignant tumors throughout the various stages of their development. In assessing the 5T4 phenotype it has been determined that there is a significant correlation between 5T4 expression and advanced stage of disease (FIGO stages III and IV) (P < 0.001) and with poorly differentiated tumors (P = 0.036) compared to well or moderately differentiated tumors. Patients with tumors expressing 5T4 were are likely to respond well to adjuvant therapy and have a significantly worse outlook in terms of survival and disease-free interval.

Purification: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide derived from human 5T4 oncofetal antigen protein

Isotype: Ig Fraction

Application Details

Application Notes: This antibody can be used for immunohistochemistry on formalin/paraffin sections (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for TPBG

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Alternative Name: 5T4 Oncofetal Trophoblast glycoprotein

UniProt: Q13641