PIWIL1 antibody (N-Term)

Catalog No. ABIN2773782

The Rabbit Polyclonal anti-PIWIL1 antibody has been validated for WB and IHC. It is suitable to detect PIWIL1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). There are 2+ publications available.

Quick Overview for PIWIL1 antibody (N-Term) (ABIN2773782)

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIWIL1 Antibody

Binding Specificity: N-Term

Sequence: FQELSLAERG GRRRDFHDLG VNTRQNLDHV KESKTGSSGI IVRLSTNHFR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against PIWIL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIWIL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 861 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIWIL1 antibody (ABIN2773782)

Tarabay, Achour, Teletin, Ye, Teissandier, Mark, Bourchis, Viville: "Tex19 paralogs are new members of the piRNA pathway controlling retrotransposon suppression." in: Journal of cell science, Vol. 130, Issue 8, pp. 1463-1474, (2017) (PubMed).

Sugimoto, Kage, Aki, Sano, Kitagawa, Nagase, Yatomi, Ohishi, Takai: "The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus." in: Biochemical and biophysical research communications, Vol. 359, Issue 3, pp. 497-502, (2007) (PubMed).

Target Details for PIWIL1

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Alternative Name: PIWIL1

Background: PIWIL1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PI
Alias Symbols: HIWI, PIWI, MIWI
Protein Interaction Partner: UBC, DICER1, TP63, TP73, TP53,
Protein Size: 861

Molecular Weight: 98 kDa

Gene ID: 9271

NCBI Accession: NM_004764, NP_004755

UniProt: Q96J94