BHMT antibody (N-Term)

Catalog No. ABIN2773785

The Rabbit Polyclonal anti-BHMT antibody has been validated for WB and IHC. It is suitable to detect BHMT in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit and Zebrafish (Danio rerio). There are 3+ publications available.

Quick Overview for BHMT antibody (N-Term) (ABIN2773785)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BHMT Antibody

Binding Specificity: N-Term

Sequence: AVEHPEAVRQ LHREFLRAGS NVMQTFTFYA SEDKLENRGN YVLEKISGQE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against BHMT. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BHMT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BHMT antibody (ABIN2773785)

Jiang, Hurt, Breen, Stabler, Allen, Orlicky, Maclean: "Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap." in: Biology open, Vol. 4, Issue 9, pp. 1154-62, (2015) (PubMed).

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Maclean, Jiang, Greiner, Allen, Stabler et al.: "Long-term betaine therapy in a murine model of cystathionine beta-synthase deficient homocystinuria: decreased efficacy over time reveals a significant threshold effect between elevated homocysteine ..." in: Molecular genetics and metabolism, Vol. 105, Issue 3, pp. 395-403, (2012) (PubMed).

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia,but such a defect has not yet been observed.
Alias Symbols: BHMT1
Protein Interaction Partner: BHMT, BBS4, BBS2, BBS1, ALG9, LAMTOR3,
Protein Size: 406

Molecular Weight: 45 kDa

Gene ID: 635

NCBI Accession: NM_001713, NP_001704

UniProt: Q93088

Pathways: Methionine Biosynthetic Process