PRP19 antibody (Middle Region)

Catalog No. ABIN2773819

This Rabbit Polyclonal antibody specifically detects PRP19 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio) and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for PRP19 antibody (Middle Region) (ABIN2773819)

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRP19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRP19 Antibody

Binding Specificity: Middle Region

Sequence: PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PRPF19. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRPF19

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 504 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRP19 antibody (ABIN2773819)

Song, Cui, Zhong, Han: "Proteomic characterisation of pancreatic islet beta-cells stimulated with pancreatic carcinoma cell conditioned medium." in: Journal of clinical pathology, Vol. 62, Issue 9, pp. 802-7, (2009) (PubMed).

Target Details for PRP19

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Alternative Name: PRPF19

Background: PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.[supplied by OMIM].
Alias Symbols: NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV
Protein Interaction Partner: HUWE1, USP4, UBE2D3, SUMO2, CEP76, CEP250, TUBGCP3, CEP57, VCP, UBC, SUMO1, NEDD8, LIN28A, EXOC7, EXOC3, RPA3, RPA2, RPA1, RNF2, SUZ12, EED, rev, MEPCE, SEC13, PARK2, TARDBP, UBD, CSNK2A2, UCHL5, WHSC1, U2AF1, PRCC, HSP90AA1, HSPB1, FN1, RBM10, vif, CDC40
Protein Size: 504

Molecular Weight: 55 kDa

Gene ID: 27339

NCBI Accession: NM_014502, NP_055317

UniProt: Q9UMS4

Pathways: Ribonucleoprotein Complex Subunit Organization