SRF antibody (N-Term)

Catalog No. ABIN2774664

This anti-SRF antibody is a Rabbit Polyclonal antibody detecting SRF in WB and ChIP. Suitable for Human, Mouse, Rat, Pig, Dog, Cow, Rabbit and Zebrafish (Danio rerio).

Quick Overview for SRF antibody (N-Term) (ABIN2774664)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-SRF Antibody

Binding Specificity: N-Term

Sequence: ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SRF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SRF

Application Details

Application Notes: WB Suggested Anti-SRF Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 508 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Background: SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Alias Symbols: MCM1
Protein Interaction Partner: Camk2d, ZIC3, ELK1, SRFBP1, SRF, NFKB1, PRKDC, CIRBP, ALDH3A1, SUMO1, UBE2I, SHBG, TRIM63, KDM6A, MYOCD, MKL1, CNTN2, CSNK2A1, SMARCA4, PELP1, KAT5, HDAC4, EPC1, SMAD7, HDAC3, MKL2, ATF6, BARX2, MAPKAPK2, FHL3, GATA4, CAMK2A, HOPX, PYCARD, NKX2-3, MINPP1,
Protein Size: 508

Molecular Weight: 52 kDa

Gene ID: 6722

NCBI Accession: NM_003131, NP_003122

UniProt: P11831